A role for the yeast PCNA unloader Elg1 in eliciting the

A role for the yeast PCNA unloader Elg1 in eliciting the

Elg1, a central player in genome stability - ScienceDirect

Jan 01, 2015 · Taken together, these results suggest a role for the Elg1-RLC in PCNA unloading, possibly during DNA replication, but more likely as a response to fork stalling or collapse. As most forms of DNA repair and damage bypass require DNA synthesis, PCNA participates in repair, and needs to be loaded and unloaded there too [64] .

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Alternative clamp loaders/unloaders | FEMS Yeast Research

Taken together, these results suggest a role for the Elg1-RLC in PCNA unloading during DNA replication. Moreover, later work (Kubota et al. 2015 ) showed that the unloading activity of Elg1 requires ligation of the newly replicated DNA, and suggested that Elg1's activity must be genome wide, and required for PCNA unloading after the Okazaki

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The importance of being modified PCNA modification and DNA

The yeast Elg1 RFC-like complex (RLC) is the principal unloader of chromatin-bound PCNA and thus plays a central role in maintaining genome stability. Here we identify a role for Elg1 in the

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Quantitative proteomic analysis of yeast DNA replication

A role for the yeast PCNA unloader Elg1 in eliciting the DNA damage checkpoint relationship between the function of Elg1/ATAD5-RLC in PCNA unloading and the …

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A role for the yeast PCNA unloader Elg1 in eliciting the

The yeast Elg1 RFC-like complex (RLC) is the principal unloader of chromatin-bound PCNA and thus plays a central role in maintaining genome stability. Here we identify a role for Elg1 in the unloading of PCNA during DNA damage. Using DNA damage checkpoint (DC)-inducible and replication checkpoint (RC)-inducible strains, we show that Elg1 is essential for eliciting the …

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Research Publication

Telomeres and stress in yeast cells: When genes and environment interact. A role for the yeast PCNA unloader Elg1 in eliciting the DNA damage checkpoint. Role of Digital Divide in the Effectiveness of e-governance: A Study on Rayagada District of Odisha .

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Eukaryotic clamp loaders and unloaders in the maintenance

Dec 18, 2020 · PCNA unloading. After completion of DNA synthesis during DNA replication and repair, PCNA is unloaded from DNA by the eukaryotic clamp unloader ATAD5-RLC (Elg1-RLC in yeast) 10,16,17,30.The RFC complex can unload PCNA in vitro 9, even though its activity is significantly lower than that of ATAD5-RLC 10.It has been reported that yeast Ctf18-RLC can …

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Alternative replication factor C protein, Elg1, maintains

The role of the Elg1-RLC in PCNA unloading appears to be conserved in humans, since ATAD5 is required for proper removal of PCNA from chromatin …

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A role for the yeast PCNA unloader Elg1 in eliciting the

Jul 22, 2019 · Elg1 forms an RFC-like complex in charge of unloading the DNA polymerase processively factor PCNA during DNA replication and DNA repair. Using checkpoint-inducible strains, a recently published paper (Sau et al. in mBio 10(3):e01159-19. https://doi.org/10.1128/mbio.01159-19, 2019) uncovered a role for Elg1 in eliciting the DNA …

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structure–function analysis of the yeast Elg1 protein

The yeast Elg1 interacts both genetically and physically with the yeast orthologs of the Fanconi Anemia repair pathway . Although it has already been established that Elg1 is an unloader of PCNA, it is still not clear whether the phenotypes of elg1Δ cells are a consequence of PCNA retention on the chromatin. To address this issue, we have used

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The Yeast PCNA Unloader Elg1 RFC-Like Complex Plays a Role

Elg1 forms an RFC-like complex in charge of unloading the DNA polymerase processively factor PCNA during DNA replication and DNA repair. Using checkpoint-inducible strains, a recently published paper (Sau et al. in mBio 10(3):e01159-19. https://doi.org/10.1128/mbio.01159-19, 2019) uncovered a role for Elg1 in eliciting the DNA damage checkpoint (DC), one of the …

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Genes | Free Full-Text | PCNA Loaders and Unloaders—One

The yeast pcna unloader Elg1 RFC-like complex plays a role in eliciting the DNA damage checkpoint. MBio 2019, 10, e01159-19. [Google Scholar] Bylund, G.O.; Burgers, P.M.J. Replication protein A-directed unloading of PCNA by the Ctf18 cohesion establishment complex. Mol. Cell.

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A role for the yeast PCNA unloader Elg1 in eliciting the

Up to10%cash back · Jul 22, 2019 · During cell proliferation, the genome is constantly threatened by cellular and external factors. When the DNA is damaged, or when its faithful duplication is delayed by DNA polymerase stalling, the cells induce a coordinated response termed the DNA damage response (DDR) or checkpoint. Elg1 forms an RFC-like complex in …

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Publications | mklab

A structure-function analysis of the yeast Elg1 protein reveals the importance of PCNA unloading in genome stability maintenance. Shemesh K, Sebesta M, Pacesa M, Sau S, Bronstein A, Parnas O, Liefshitz B, Venclovas C, Krejci L, Kupiec M. Nucleic Acids Res. 2017 Apr 7;45(6):3189-3203. doi: 10.1093/nar/gkw1348. PMID:27834202 | PMCID:PMC5101353

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Eukaryotic clamp loaders and unloaders in the maintenance

Dec 18, 2020 · Eukaryotic sliding clamp proliferating cell nuclear antigen (PCNA) plays a critical role as a processivity factor for DNA polymerases and as …

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Proceedings of the 2005 IEEE International Conference on

Equations of Motion of a Single-Wheel Robot in a Rough Terrain Aria Alasty, Hodjat Pendar. 879-884; Model Predictive Control for Vehicle Guidance in Presence of Sliding: Application to Farm Vehicles Path Tracking Roland Lenain, Benoit Thuilot, …

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The PCNA-RFC families of DNA clamps and clamp loaders

A role for the yeast PCNA unloader Elg1 in eliciting the DNA damage checkpoint. 2019) uncovered a role for Elg1 in eliciting the DNA damage checkpoint (DC), one of …

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Pivotal roles of PCNA loading and unloading in

Feb 27, 2018 · PCNA is regulated, in part, by controlling when and where it resides on chromatin through the combined actions of two five-membered protein complexes that load and unload PCNA onto DNA ( 45 ). The RFC complex consisting of Rfc1–5 loads PCNA, and the other complex, Elg1–Rfc2–5, unloads PCNA ( 45 ⇓⇓⇓ – 49 ).

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Is PCNA unloading the central function of the Elg1/ATAD5

PCNA Unloading by S. cerevisiae Elg1-RLC Several lines of evidence from yeast suggested a role for Elg1-RLC in PCNA regulation during DNA replication.6,29,36 Martin Kupiec's group demonstrated that yeast Elg1-RLC preferentially interact with SUMOylated PCNA, and in cells lacking Elg1, they found SUMOylated PCNA accumulated on chromatin in

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Replication-Coupled PCNA Unloading by the Elg1 Complex

function of S. cerevisiae Elg1-RLC is unloading of PCNA during DNA replication (Kubota et al., 2013a, 2013b). The function of the Elg1-RLC in PCNA unloading appears to be conserved in humans, since ATAD5 (the human Elg1 homolog) is required for proper removal of PCNA from chromatin in human cell lines (Lee et al., 2013; Shiomi and Nishitani, 2013).

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